Vol. 7, Special Issue 6 (2019)
Mutation frequency, spectrum of induced mutagenesis in M4 generation of soybean (Glycine max L. Merrill) genotypes
Author(s): Priyanka Kumari, M Chakraborty, KP Prasad, Sumit Kumar Mishra and Priyadarshni Raj
Abstract: Soybean is self pollinated crop having small and fragile flowers that’s why its very difficult to hybridization programme so, here Mutation breeding used here for crop improvement. The practical role of induced mutation in the improvement of crop plants can best be assessed on the basis of qualitatively inherited characters. Induced mutagenesis plays an important role in improvement of crops soybean 300 (each) Representative M4 Gamma irradiated seeds of two varieties of soybean, BSS-2 and RKS-18 procured from the Department of Genetics and Plant Breeding, BAU, Ranchi were sown in kharif 2017. Seeds of both the varieties viz. BSS-2 and RKS-18 were exposed to five different doses of gamma rays (50 Gy, 100 Gy, 150 Gy, 200 Gy and 400 Gy) in the year 2014 and 2015 using Cobalt 60 sources in Gamma chamber at Bhabha Atomic and Research Centre, Mumbai and their M3 progenies were used as experimental materials. Data were recorded for lethality percentage, (15 days after sowing and at flowering) injury percentage in untreated control as well as treated plants. Mutation rate, effectiveness, frequency of five different doses of gamma rays (50 Gy, 100 Gy, 150 Gy, 200 Gy and 400 Gy) and spectrum of qualitative mutations of both the variety in BSS-2 and RKS-18 was studied in the M4 generation. Mutation rate for BSS-2 (0.0596) and for RKS-18 (0.0682) mutation frequency is equal for 50 Gy (8) and 100 Gy (8) in BSS-2 and in RKS-18 in 50 Gy found highest (10). Effectiveness was highest in 50 Gy (0.16), (0.2) in both BSS-2 RKS-18.
Pages: 601-604 | 427 Views 58 Downloads
download (4465KB)
How to cite this article:
Priyanka Kumari, M Chakraborty, KP Prasad, Sumit Kumar Mishra and Priyadarshni Raj. Mutation frequency, spectrum of induced mutagenesis in M4 generation of soybean (Glycine max L. Merrill) genotypes. International Journal of Chemical Studies. 2019; 7(6): 601-604.
