Vol. 7, Special Issue 6 (2019)
Double haploid production & breeding approaches
Author(s): Shreya Sen, Kusumakar Gautam and Sandeep Kumar Yadav
Abstract: Doubled haploid (DH) production has become an important tool in plant breeding largely due to its capacity to produce completely homozygous plants in one generation. Doubled haploids (DHs) are plants derived from a single pollen grain and doubled artificially to form homozygous diploids. A DH individual has two identical homologs, so that the amount of recombination information is equivalent to a backcross. Homozygosity at a particular locus is always desirable feature for crop improvement programs. Breeding is one of the approaches to attain this but it requires minimum 6-7 cropping years. Use of haploid to produce double haploid is one of the potent approaches to overcome this lacuna. Haploid can be either produce through androgenesis or gynogenesis, androgenesis being most used approach. Not only are traits fixed for selection but the multiple generations of inbreeding required using traditional breeding methods are circumvented. However, prior to implementing a DH breeding program, the breeder should consider factors such as the potential for linkage drag, types of crosses to be used and whether production resources are sufficient to produce the DH populations necessary for success. Doubled haploid technology can be integrated with marker-assisted breeding for greater efficiency and to craft the DH population for particular traits. The technology can also be used to accelerate development of germplasm with new genes of interest and to generate cytogenetic stocks. The future for DH breeding is promising because robust DH protocols are available for an ever-growing number of crops and future applications will see a closer integration with molecular-marker and gene-splicing technologies
Pages: 319-324 | 504 Views 72 Downloads
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How to cite this article:
Shreya Sen, Kusumakar Gautam and Sandeep Kumar Yadav. Double haploid production & breeding approaches. International Journal of Chemical Studies. 2019; 7(6): 319-324.
