Vol. 12, Issue 5 (2024)
Molecular characterization of black pepper (Piper nigrum L.) genotypes using ISSR markers
Author(s): Sudheesh Kulkarni, NK Hegde, Laxminarayan Hegde, Vijayakumar Narayanpur, Mukesh Chavan, Sadananda GK, Prashant A and Mahantesh Naik
Abstract: Black pepper is a perennial glabrous woody climber belonging to family piperaceae, has a major contribution in Indian Spice Export Scenario. Black pepper is one of the oldest spices known to mankind and used since prehistoric times. The genetic diversity is normally distinguished through morphological characters. However, such morphological characters could be affected by environmental influences, developmental stage of the plant, type of planting material evaluated, management practices, etc. DNA markers like RAPD and ISSR are most suitable for exact genetic diversity analyses, as these markers are not altered for the environment. In this connection, the present study was conducted with an objective to characterise the black pepper genotypes using 5 ISSR markers. The primer ISSR 2 used for molecular chararcterisation is found to be effective in showing the genetic diversity and polymorphic character among the fifty two genotypes studied. The constructed dendrogram grouped the 52 genotypes into two major clusters. Cluster I consist of 48 genotypes, while Cluster II comprised 4 genotypes. It confirms variation in the local genotypes of Uttara Kannada District, Karnataka.
Pages: 15-18 | 217 Views 101 Downloads
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How to cite this article:
Sudheesh Kulkarni, NK Hegde, Laxminarayan Hegde, Vijayakumar Narayanpur, Mukesh Chavan, Sadananda GK, Prashant A, Mahantesh Naik. Molecular characterization of black pepper (Piper nigrum L.) genotypes using ISSR markers. Int J Chem Stud 2024;12(5):15-18.
