Vol. 12, Issue 4 (2024)

In quest for biologically more potent compounds, we envisioned synthesizing series of hybrids of alkyl/aryl urea and 6-methoxy-2-aminobenzothiazole by the treatment of 6-nethoxy-2-aminobenzothiazole with phenylchloroformate using anhydrous pyridine in dry THF at room temperature (RT) initially to obtain corresponding isocyanates. The resultant isocyanate is further refluxed for 10-12 hrs with monoalkyl/aryl urea derivatives in presence of sodium hydride (NaH) in dry THF. The synthesized biuret compounds were characterized by 1HNMR and Rf values, and subsequently evaluated for antibacterial activity against Staphylococcus aureus, Escherichia coli, Klebsiella pnemoniae, and Pseudomonas aeruginosa, as well as antifungal activity against Aspergillus Niger, Aspergillus flavus, and Fusarium monoliforme. Among the synthesized compounds, some of the biurets demonstrated significant activity, while rest of the biuret compounds exhibited moderate activity.