Vol. 11, Issue 2 (2023)

The biosynthesis of nanoparticles was performed using Trichoderma as a reducing and stabilizing agent. Isolated nanoparticles were characterized by UV‒VIS spectrophotometry and TEM. Antifungal potential of the nanoparticles studied on seed-borne pathogenic microflora of rice. Antifungal assays were performed. In vitro, by the well diffusion method using media supplemented with 100-500 ppm NP solution and amp, growth inhibition was calculated, and the degree of sensitivity was studied on the basis of the lethal concentration (LC) value and statistical analysis. Study shows that AgNp inhibits growth in Fusarium fujikuroi, Aspergillus flavus, and Curvularia lunata at concentrations ranging from 100 to 500 ppm. In Aspergillus, however, the percentage is 65.54%. LC 50 analysis shows that the LC 50 value is between concentrations of 100 ppm and 300 ppm, which is optimized up to 140 ppm. This study also concluded that silver nanoparticles are much more applicable as antifungal and antibacterial agents and are more diverse in function.