Vol. 10, Issue 4 (2022)
Bio-Synthesis of silver nanoparticles using a food colorant produced by Monascus purpureus MTCC 410 and its inhibitory effect on the food-borne human pathogen, Aspergillus flavus
Author(s): Swati Padmakar Bhavsar
Abstract: Aflatoxins are secondary metabolites produced by species of Aspergillus fungus with toxic, carcinogenic, mutagenic, and teratogenic effects. Aspergillus spp. often grows on the food commodities such as peanuts, rice, corn and cottonseed, so contamination of these commodities with aflatoxin makes them unsuitable for human and animal consumption. With the aim to inhibit the growth of mycotoxigenic fungus Aspergillus flavus NCIM 538, silver nanoparticles were biologically synthesized using a red pigment produced by Monascus purpureus MTCC 410 (the Red Yeast Rice Fungus). The red pigment production was studied in the cooked rice and jack fruit seed powder medium by solid state fermentation. The extracellular pigment was effectively extracted using one step method by treating it with 95% ethanol followed by filtration and air drying. The pigment was characterized using the chromatographic and spectrophotometric method. The silver nanoparticles (AgNPs) synthesized using the red pigment were characterized by UV Spectrophotometry, transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). TEM images showed that the generated nanoparticles were varied in size (20-50 nm) and shape, and exhibited a surface plasmon resonance at around 437 nm. The inhibitory effect of AgNPs was assessed against Aspergillus flavus NCIM 538 at various concentrations (10 µg/ml-100 mg/ml). The fluorescence of the aflatoxin was observed to decrease with increase in the concentration of AgNPs which indicated the inhibitory effect of AgNPs on growth of Aspergillus flavus and aflatoxin production. The biological property of AgNPs can be effectively altered to work at a lesser concentration by manipulating its physical and biochemical properties.
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How to cite this article:
Swati Padmakar Bhavsar. Bio-Synthesis of silver nanoparticles using a food colorant produced by Monascus purpureus MTCC 410 and its inhibitory effect on the food-borne human pathogen, Aspergillus flavus. Int J Chem Stud 2022;10(4):01-06.
