International Journal of Chemical Studies
Vol. 10, Issue 2 (2022)
Preparation and characterization of streptavidin-biotin aptamer coated magnetic nanoparticle for colour based detection system of antibiotic residues in biological samples
Author(s): Kalaiselvi G, Tirumurugaan KG, Dhinakar Raj G, Vijayarani K and Baranidharan R
Abstract: Aptamers are oligonucleotides that bind to a specific target molecule. Nucleic acid aptamers are usually created by selecting them from a large random sequence pool. Mostly magnetic nanoparticles are inert in nature, low toxicity and biocompatible. Functionalization of magnetic particle with an existing surface and gives a new surface with possibly new properties. Magnetic particle prepared by chemical co precipitation technique of iron salts in aqueous medium. The zeta value of -6.5Mv before coating with aptamer and 35.6 Mv after coating with OTC specific aptamer. The nanoparticle size shows 155.4nm before coating with OTC specific aptamer and 446.1nm after coating with aptamer. In case of coating, 250 µg of magnetic nanoparticle and 300 µg of streptavidin concentration shows good peroxidase activity. Polymerase chain reaction of oxytetracycline aptamer with specific primer shows amplification size 76 bp at 4% agarose gel in aptamer coated particle but negative in uncoated particle. Washing and storing of aptamer coated particle does not remove the aptamer from the surface of magnetic particle which indicates the aptamer and magnetic coupling is more stable. The DNA content of supernatant and coated magnetic particles were analysed by Nano drop which shows the coated magnetic particle have higher DNA content when compared with supernatant.
Pages: 11-16 | 80 Views 15 Downloads
How to cite this article:
Kalaiselvi G, Tirumurugaan KG, Dhinakar Raj G, Vijayarani K, Baranidharan R. Preparation and characterization of streptavidin-biotin aptamer coated magnetic nanoparticle for colour based detection system of antibiotic residues in biological samples. Int J Chem Stud 2022;10(2):11-16.