International Journal of Chemical Studies
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P-ISSN: 2349-8528, E-ISSN: 2321-4902   |   Impact Factor: GIF: 0.565

Vol. 10, Issue 1 (2022)

In vitro evaluation of fungicides against diplocarpon rosae causing black leaf spot in rose


Author(s): Ashwini Matte, MJ Patil, RB Kothikar, Tini Pillai and HR Sawai

Abstract: The rose leaves showing typical symptoms of black spot were collected from Horticulture Field, College of Agriculture, Nagpur and used for isolation of pathogen on potato dextrose agar media. The culture of the pathogen was examined for morphological and cultural characteristics. On the basis of hyphae, shape of spore produced, colour and type of colony, the fungus was identified as Diplocarpon rosae and it proved the Koch’s postulates. The fungicides viz., Azoxystrobin (Amistra), Mancozeb+Carbendazim (Bendaco), Thiophenate methyl (Topsin-M), Mycobutanil (Systhane), Tebuconazole (Folicure) and Chlorothalonil (Daconil) were evaluated against Diplocarpon rosae in-vitro by poisoned food technique. Results revealed that, all the fungicides significantly inhibited the growth of Diplocarpon rosae over untreated control. Chlorothalonil 75 WP @ 2% and Topsin M 70 WP @ 0.05% showed minimum colony diameter of 8.33 mm and 16.45 mm respectively and found significantly superior over all the test fungicides. Inhibition of growth varies from 48.14 to 90.74 per cent. Highest inhibition was observed by Chlorothalonil 75 WP @ 2% (90.74%) and Topsin M 70 WP @ 0.05% (81.72%).

Pages: 35-39  |  511 Views  73 Downloads

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How to cite this article:
Ashwini Matte, MJ Patil, RB Kothikar, Tini Pillai, HR Sawai. In vitro evaluation of fungicides against diplocarpon rosae causing black leaf spot in rose. Int J Chem Stud 2022;10(1):35-39.
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