Vol. 9, Issue 3 (2021)
Molecular variability among different isolates of Fusarium solani causing root rot in chickpea by SSR marker
Author(s): Priyanka Lohar, SS Mane and Puja Walunj
Abstract: The Genetic diversity of all the isolates of Fusarium solani was analysed by using Simple Sequence Repeat (SSR) markers. The SSR primers were tested for amplification of genomic DNA of Fusarium solani isolates. The SSR analysis total 6 primers were screened against six isolates of Fusarium solani i.e. Fs1, Fs2, Fs3, Fs4, Fs5 and Fs6. Out of 6 primers 3 primers amplified scorable banding pattern. In which 6 SSR primer produced 41 scorable bands with 80.48% level of polymorphism. In the dendrogram two major clusters were obtained on the basis of analysis Cluster A and Cluster B. Cluster A include Fs4 and Fs6. Cluster B include Fs1, Fs2, Fs3 and Fs5. The similarity matrix indicated that six isolates of Fusarium solani exhibited in between 0.880 to 0.323 percent similarity coefficients for SSR primer. The results show that SSR analysis were suitable for the evaluation of genetic polymorphisms among fungal populations.
Pages: 241-245 | 448 Views 81 Downloads
download (4400KB)
How to cite this article:
Priyanka Lohar, SS Mane, Puja Walunj. Molecular variability among different isolates of Fusarium solani causing root rot in chickpea by SSR marker. Int J Chem Stud 2021;9(3):241-245.
