International Journal of Chemical Studies
Vol. 9, Issue 2 (2021)
Assessment of biomolecules and enzymes during drought stress in Echinochloa frumentacea grown in Himalaya
Author(s): Dipti Singh, Ashutosh Dubey and AK Verma
Abstract: The effect of drought stress in barnyard millets (Echinochloa frumentacea) varieties viz VL29, VL207, VL172 and VL181 was studied under in vitro environment in presence of PEG 6000 in liquid MS medium with different osmotic potential 5% w/v (-0.5MPa), 10% w/v (-0.15MPa), 15% w/v (-0.30MPa) and 20% w/v (-0.49MPa). Biochemical and enzymatic parameters were assessed in 12 days old seedling imposed to various concentration of PEG and evaluated every 24 hrs up to 7 days. Parameters of oxidative stress malondialdehyde (MDA), and hydrogen peroxide (H2O2) concentration, activities of stress responsive enzymes ascorbate peroxidase (APX), guaiacol peroxidase (GPX), catalase (CAT) and superoxide dismutase (SOD) and antioxidant molecules ascorbic acid (ASC) and proline were determined. Maximum response to drought stress was alleviated at 20% w/v (-0.49MPa) PEG as compared to that of control. However variety VL172 responded better among the other three varieties. The relative drought tolerant ability associated with variety VL172 due to high antioxidant enzyme activity, high proline and ascorbic acid content with low MDA content and H2O2 content. While variety VL29 was found to susceptible to drought stress. Due to high content of MDA and H2O2 and lower antioxidant activities was found in variety VL29. Data of this study clearly suggest the mechanism to limit the oxidative damaged which is important for drought tolerance for variety VL172 among the four varieties of barnyard millet.
Pages: 29-52 | 93 Views 3 Downloads
How to cite this article:
Dipti Singh, Ashutosh Dubey, AK Verma. Assessment of biomolecules and enzymes during drought stress in Echinochloa frumentacea grown in Himalaya. Int J Chem Stud 2021;9(2):29-52.