Vol. 8, Issue 6 (2020)
Isolation and characterisation of sulphur oxidising bacterial isolates from two different pacific white shrimp, Penaeus vannamei culture systems
Author(s): Hareshkumar G Solanki, Natesan Manoharan, Trupti K Vyas, Govindan Rajivgandhi, Krishna Patel and Prasanna Kumar Patil
Abstract: Sulphur reducing bacteria (SRB) and sulphur-oxidizing bacteria (SOB) are predominately involved in reduction and oxidation of sulphates and hydrogen sulphide, respectively, in pond and assist in maintaining healthy pond environment for shrimp culture. Hence, the present study aims to find out SOB two different Penaeus vannamei culture systems. Total 43 isolates from earthen ponds and 38 from plastic-lined ponds were obtained using thiosulphate agar and Starkey medium. The isolates were tested for their efficacy to reduce the pH of the growth media from 8.0 to ≤5.0 within 15 days of incubation. Data revealed the sulphate production was in the range of 2.5 to 195.36 mg/100 ml. The bacterial isolates from earthen pond showed significantly higher (P< 0.05) sulphate ion production compared to bacteria isolated from the plastic-lined pond. Among all these isolated bacteria, fifteen isolates possess autotrophic metabolism. SOBs were able to tolerate salt up to 13%. Morphological and biochemical characterization of isolated SOB revealed that as Bacillus species and Micrococcus sp. were present in the plastic-lined pond isolates whereas, Bacillus sp., Geobacillus sp., Amphibacillus sp., Anoxibacillus sp. and Alkalibacillus sp. were present in the earthen ponds. Thus, the earthen pond has more diverse SOB than in the plastic-lined pond.
Pages: 39-45 | 558 Views 38 Downloads
How to cite this article:
Hareshkumar G Solanki, Natesan Manoharan, Trupti K Vyas, Govindan Rajivgandhi, Krishna Patel, Prasanna Kumar Patil. Isolation and characterisation of sulphur oxidising bacterial isolates from two different pacific white shrimp, Penaeus vannamei culture systems. Int J Chem Stud 2020;8(6):39-45. DOI: 10.22271/chemi.2020.v8.i6a.11173