Vol. 8, Issue 5 (2020)
A review on detection and diagnosis technique of tobacco streak virus (TSV) infecting field and horticultural crops
Author(s): Kota Venkata Ramesh and S Abdul Salam
Abstract: Tobacco Streak Virus causes severe losses in many field and horticultural crops. The control of TSV depends on the accurate and timely diagnosis. Most of the routine TSV diagnosis assays are based on ELISA with either polyclonal or monoclonal antibodies. Detection and diagnosis of TSV has been mainly based on biological indicators, Electron microscopy, serological methods and RT-PCR. Indirect-ELISA using polyclonal antibodies raised against whole virion was used for the detection of TSV isolates in infected plant extracts. RT-PCR using TSV-specific PCR primers flanking the coat protein gene were used to amplify corresponding sequence from leaf samples of different crop plants. IC-RT-PCR based detection of TSV in plant samples is advantages over other well established virus detection approaches, such as ELISA or regular RT-PCR, because it combines the ELISA characteristic of good adaptation to routine automatic analysis, with the superior sensitivity of PCR amplification of nucleic acids, avoiding the need for nucleic acid purification steps.
DOI: 10.22271/chemi.2020.v8.i5ak.12518
Pages: 2750-2754 | 106 Views 61 Downloads
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How to cite this article:
Kota Venkata Ramesh, S Abdul Salam. A review on detection and diagnosis technique of tobacco streak virus (TSV) infecting field and horticultural crops. Int J Chem Stud 2020;8(5):2750-2754. DOI: 10.22271/chemi.2020.v8.i5ak.12518
