Vol. 8, Issue 5 (2020)
Effect of milk fermented with Lactobacillus sp. in modulation of intestinal permeability in high fat diet fed mice
Author(s): Raghvendra Kumar, Rajkumar Sharma, Surendra Jangra and Gaurav Bhakri
Abstract: Consumption of high fat diet (HFD) is known to disturb the gut microflora, which is a source of endotoxins. Its increase in plasma is related to increased intestinal permeability. In the present study, effect of milk fermented with L. casei NCDC-19 in reference to intestinal permeability/integrity was evaluated using Swiss Albino mice. Permeability was determined using FITC-dextran 4000 Da through measurement of fluorescence in blood plasma. HFD feeding resulted in a significant increase in intestinal permeability. A positive effect of dietary supplementation of probiotic fermented milk on intestinal permeability was observed. Expression of two important genes related to intestinal permeability was determined by qRT-PCR. Reduced expression of tight junction proteins was observed in HFD fed group which confirms the increased permeability. Occludin was found up-regulated significantly as result of probiotic fermented milk. A similar trend of up-regulation of ZO-1 was seen in probiotic FM/skim milk fed groups. Present study suggests a positive effect of probiotic fermented milk on intestinal permeability in HFD fed conditions.
DOI: 10.22271/chemi.2020.v8.i5aj.10710
Pages: 2600-2604 | 555 Views 129 Downloads
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How to cite this article:
Raghvendra Kumar, Rajkumar Sharma, Surendra Jangra, Gaurav Bhakri. Effect of milk fermented with Lactobacillus sp. in modulation of intestinal permeability in high fat diet fed mice. Int J Chem Stud 2020;8(5):2600-2604. DOI: 10.22271/chemi.2020.v8.i5aj.10710
