Vol. 8, Issue 4 (2020)
Comparision of iron-regulated outer membrane proteins (IrOMP) and iron-sufficient outer membrane proteins (IsOMP) of Pasteurella multocida strains of porcine origin
Author(s): Purabi Deka, RK Sharma and N Pathak
Abstract: An investigation was carried out to compare the effect of growth of P. multocida type A of pig origin in iron restricted and in iron sufficient media on the basis of their outer membrane protein extract. Pasteurella multocida serotype A was cultured in two ways. In one process only BHI medium was used and on the other process same medium was used which was supplemented with 2, 2’- Dipyridyl (iron-restricted medium). Both these cultures were used to obtain Outer Membrane Proteins (OMPs) by extraction of bacterial cells with 1% Sarcosyl. Separation of the OMPs in SDS-PAGE showed that OMPs were mixture of protein fraction with molecular weight ranging from 110 to 22.6 kDa in case of iron-restricted OMP and 47.3 to 29.9 kDa in case of iron sufficient OMP. The OMP with molecular masses 29.9 kDa grew in both iron-restricted as well as in iron sufficient medium.
DOI: 10.22271/chemi.2020.v8.i4aq.10176
Pages: 3402-3404 | 490 Views 115 Downloads
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How to cite this article:
Purabi Deka, RK Sharma, N Pathak. Comparision of iron-regulated outer membrane proteins (IrOMP) and iron-sufficient outer membrane proteins (IsOMP) of Pasteurella multocida strains of porcine origin. Int J Chem Stud 2020;8(4):3402-3404. DOI: 10.22271/chemi.2020.v8.i4aq.10176
