Vol. 7, Issue 5 (2019)
Optimization of process parameters through plackett-burman design by Paenibacillus polymyxa G7 isolated from resins of Pinus roxburghii
Author(s): Ranjana Sharma and Nivedita Sharma
Abstract: Lipases are ubiquitous in nature and high demanded industrial catalysts. In the present study, the cultural conditions of extracellular lipase production from a bacterial strain Paenibacillus polymyxa G7 were statistically optimized by using Plackett-Burman design under submerged fermentation conditions. Maximum enzyme activity was noted in a medium containing; galactose (0.2%), ammonium sulphate (0.5%), Tragacanth gum (1.5%), yeast extract (0.25%), incubation time (4 days), temperature (40 ºC), pH 10.0 with an inoculum size 10%. As witnessed from Pareto chart; variables that were most important for lipase production were temperature and incubation time (days) whereas other parameters showed minute effect for lipase production.
Pages: 726-731 | 485 Views 143 Downloads
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How to cite this article:
Ranjana Sharma, Nivedita Sharma. Optimization of process parameters through plackett-burman design by Paenibacillus polymyxa G7 isolated from resins of Pinus roxburghii . Int J Chem Stud 2019;7(5):726-731.
