Vol. 7, Issue 4 (2019)
Molecular characterization of eukaryotic translation initiation factor eIF4E gene in PRSV resistant highland papaya
Author(s): Harini V, Varanavasiappan S, Arul L, Kokiladevi E, Sudhakar D, Sooriyanathasundaram K and Kumar KK
Abstract: The eukaryotic translation initiation factor eIF4E gene was isolated from PRSV resistant highland wild papaya, Vasconcellea cauliflora. The eIF4E gene comprises of four exons separated by three intronic regions. Gene specific primers were designed in the conserved region of eIF4E gene, to isolate it from V. cauliflora. The isolated eIF4E cDNA of V. caulifloraencoded a 237 amino acid polypeptide, which was aligned with eIF4Eprotein from three PRSV resistant Vasconcellea species (V. cundinamarcensis, V. goudotiana, V. monica), two PRSV susceptible Vasconcellea species (V. parviflora, V. querciflora)and papaya. Multiple sequence alignment between PRSV susceptible and resistant species revealed two common amino acid substitutions at L227H and S230G. The eIF4Einteracts with viral genome-linked protein (VPg) at 5’ cap of viral mRNA, which is necessary for viral protein translation. These amino acid substitutions in eIF4E protein of PRSV resistant Vasconcellea spp. might be responsible for host resistance to potyvirus.
Pages: 411-415 | 625 Views 168 Downloads
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How to cite this article:
Harini V, Varanavasiappan S, Arul L, Kokiladevi E, Sudhakar D, Sooriyanathasundaram K, Kumar KK. Molecular characterization of eukaryotic translation initiation factor eIF4E gene in PRSV resistant highland papaya. Int J Chem Stud 2019;7(4):411-415.
