Vol. 7, Issue 4 (2019)
Differentiation of sheep and goat species by PCR-RFLP of mitochondrial 12S rRNA gene
Author(s): Deep Prakash Saikia, DJ Kalita, Krishna Bharali and P Borah
Abstract: The present study was carried out with an aim to develop a method for differentiation of sheep and goat meat using PCR-RFLP. Tissue samples were collected randomly from both the species and used for mitochondrial DNA extraction. PCR amplification of a partial 456bp fragment of 12S rRNA gene was done using universal primer for both the species. The PCR-RFLP studies on 12S rRNA gene in goat and sheep using BspTI and ApoI restriction enzymes respectively, lead to production of 323bp and 133bp fragment in goat and 320bp and 136bp fragments in sheep. The resulting RFLP pattern of 12S rRNA could easily identify and differentiate meat of sheep and goat species.
Pages: 132-135 | 602 Views 45 Downloads
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How to cite this article:
Deep Prakash Saikia, DJ Kalita, Krishna Bharali, P Borah. Differentiation of sheep and goat species by PCR-RFLP of mitochondrial 12S rRNA gene. Int J Chem Stud 2019;7(4):132-135.
