Vol. 7, Issue 3 (2019)
In vitro propagation of lily (Lilium longiflorum T.) cv. Pavia in continuous immersion bioreactor (CIB)
Author(s): Narendra Singh Bhandari, C Aswath and L Reddy
Abstract: In the global flower industry, lily (Lilium longiflorum T.) holds 4th position among top 10 cut flowers and 2nd among geophytes. Conventional micropropagation technique using solid media is a typically labor-intensive method of producing elite clones of lily cultivars. Commercially, micropropagation is more important than seed or vegetative propagation. The present investigation was conducted to assess the viability of Continuous immersion bioreactor (CIB) for development of lily bulbs in vitro. To establish the protocol, we used in vitro regenerated bulblets of Lilium cv. Pavia as starting material. Factors such as additional sucrose, duration of subculture and ventilation were tested. Total fresh weight and bulb size was significantly increased by media refreshment. The sucrose supplementation in MS media also plays a crucial role in growth and development of lilium bulbs. Larger bulblets were formed in MS liquid media supplemented with 9% sucrose. The ideal bulb size (>32.83 mm in circumferences) and weight (>23.76 g) was achieved when bulblets were cultured in CIB for 12 weeks with 4 times media renewal at 2 weeks interval. More than 90% of plantlets survived in a cocopeat and soil (1:1) substrate. This protocol allows the production of lily bulbs in a simple CIB, with effective rooting and acclimatization. This is the first CIB mediated protocol for lilium bulb production in India.
Pages: 5132-5134 | 360 Views 99 Downloads
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How to cite this article:
Narendra Singh Bhandari, C Aswath, L Reddy. In vitro propagation of lily (Lilium longiflorum T.) cv. Pavia in continuous immersion bioreactor (CIB). Int J Chem Stud 2019;7(3):5132-5134.