Vol. 7, Issue 3 (2019)
Genetic diversity among castor (Ricinus communis L.) genotypes as revealed by RAPD and ISSR markers
Author(s): Harshita J Kachhadiya, RB Madaria, Rushita V Bhadani, Abhijeeta K Nandha, Manthan Kapuria and HP Gajera
Abstract: Castor (Ricinus communis L.) is an important plant for production of industrial oil. The systematic evaluation of the molecular diversity encompassed in castor inbreds or parental lines offers an efficient means of exploiting the heterosis in castor as well as for management of biodiversity. Two DNA-based molecular marker techniques, viz., random amplified polymorphism DNA (RAPD) and inter simple sequence repeat (ISSR), were used to assess the genetic diversity in castor genotypes. Out of the 40 RAPD and 40 ISSR primers screened, a total of 35 polymorphic primers (24 RAPDs and 23 ISSRs), were used in this study. Amplification of genomic DNA of 20 castor genotypes, using RAPD analysis, yielded 147 fragments, of which 96 were polymorphic, with an average of 4.0 polymorphic fragments per primer. Number of amplified fragments with RAPD primers ranged from 2 to 13, with the size of amplicons ranging from 102 to 2548 bp in size. The polymorphism ranged from 0.0 to 100.0, with an average of 66.68%. The 23 ISSR primers produced 142 bands across 20 genotypes, of which 118 were polymorphic, with an average of 5.13 polymorphic fragments per primer. The number of amplified bands varied from 2 to 11, with size of amplicons ranging from 100 to 2342 bp. The percentage of polymorphism using ISSR primers ranged from 0.0 to 100.0, with an average of 78.54%. The Mantel test between the two Jaccard’s similarity matrices gave r = 0.78, showing correlation between RAPD- and ISSR-based similarities. Clustering of genotypes within the groups was not similar when RAPD and ISSR derived dendrograms were compared, whereas, the pattern of clustering of the genotypes remained akin in RAPD and combined data of RAPD and ISSR. The similarity coefficient ranged from 0.58 to 0.88, 0.41 to 0.77, and 0.69 to 0.93 with RAPD, ISSR, and combined dendrogram, respectively. Knowledge on the genetic diversity of castor can be used to future breeding programs for increased oil production to meet the ever increasing demand of castor oil for industrial uses as well as for biodiesel production.
Pages: 2625-2633 | 360 Views 87 Downloads
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How to cite this article:
Harshita J Kachhadiya, RB Madaria, Rushita V Bhadani, Abhijeeta K Nandha, Manthan Kapuria, HP Gajera. Genetic diversity among castor (Ricinus communis L.) genotypes as revealed by RAPD and ISSR markers. Int J Chem Stud 2019;7(3):2625-2633.
