International Journal of Chemical Studies
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P-ISSN: 2349-8528, E-ISSN: 2321-4902   |   

Vol. 7, Issue 3 (2019)

Molecular characterization of exon-2 of buffalo mammocytes defensin for exploring its potency for synthesis of novel antimicrobial agents


Author(s): DJ Kalita, J Barkakati, D Rajkhowa, M Hazarika and A Kumar

Abstract: Antibiotic resistant is a serious concern for both veterinary and medical practices and to trounce these problems there is a need to search alternate group of drugs for prevention and treatment of different diseases. Mammalian defensin and cathelicidin are the two broad classes of antimicrobial peptides expressed by different epithelial lining of the living organisms. Present study was designed to characterize the buffalo mammocytes defensin to find out the template for synthesis of novel antimicrobial agents. Genomic DNA was isolated from buffalo mammocyte. The amplified PCR product was purified cloned and sequenced. The size of the PCR product was 263 bp and cloned DNA after sequencing revealed that exon-2 of the mammocyte defensin is comprised of 129 bases. The total number of predicted amino acids was 42 and the aligned amino acid sequences of buffalo mammocyte defensin with other defensin peptides showed six conserved cysteine residue at different position. The mature peptide had six strongly basic and eleven hydrophobic amino acids including one tryptophan in all most at middle of the peptide. From the present study, it can be concluded that the buffalo mammocyte defensin can act as blue print for synthesis of new antimicrobial agents.

Pages: 1868-1871  |  457 Views  72 Downloads

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How to cite this article:
DJ Kalita, J Barkakati, D Rajkhowa, M Hazarika, A Kumar. Molecular characterization of exon-2 of buffalo mammocytes defensin for exploring its potency for synthesis of novel antimicrobial agents. Int J Chem Stud 2019;7(3):1868-1871.
 

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