Vol. 7, Issue 3 (2019)
Evaluation of genetic diversity of Jatropha genotypes (Jatropha curcas L.) by using RAPD molecular marker
Author(s): Bhawesh Joshi, MK Nautiyal, Leela Bhatt and Vikas Mangal
Abstract: The genetic diversity in Jatropha curcas was evaluated through RAPD marker (Random Amplified Polymorphic) in a diverse, local, panel of 25 genotypes. Out of ten RAPD primers, five showed null result, and only five were found to be polymorphic. A total of 47 marker levels were amplified that exhibited 55.39 percent polymorphism. The highest 7 bands were observed for primers OPA-02 and 6 bands for OPAW-07.The average percentage number of monomorphic bands per primer was 30 and the percentage of polymorphism ranges from 44.44(OPA-04) to 62.50(OPH09). The Jaccard`s similarity coefficient range was found to vary from 0.80 to 1.0 i.e. 80 to 100 percent. The highest value for genetic similarity (1.0) was found between Sagar (SFRI, Jabalpur) and Kaladungi rd-15, PKVJ-MKV-1 and Lower Sowan Cheack, Danibunger-28 and Low Dhearti, Danibunger-27 and Low Dhearti. The lowest value for the similarity coefficient (0.80) was found between Pant J. Sel-1 and PKVJ-MKV-1, Pant J. Sel-1and Kamaluaganja-24, Pant J. Sel-1and Lamachaur-5, Pant J. Sel-1 and Lower Sowan Cheack, Pant J. Sel-1and Lamachaur-3. The crosses among the genotypes showing lower value of genetic similarity would be more useful for plant breeding purposes rather than those genotypes having high genetic similarity. All the loci were amplified by the RAPD primer which were found to be polymorphic varied in size from 100 bp to 1500 bp. UPGMA (Unweighted Pair Group Method with Arithmetic Mean) ordered the populations of 25 genotypes into single cluster A divided into three clusters. With cluster highest number of accession grouped under cluster 1.Lamachaur 5 showed maximum diversity with all other genotypes.it showed 15 per cent diversity.
Pages: 504-508 | 553 Views 100 Downloads
download (6827KB)
How to cite this article:
Bhawesh Joshi, MK Nautiyal, Leela Bhatt, Vikas Mangal. Evaluation of genetic diversity of Jatropha genotypes (Jatropha curcas L.) by using RAPD molecular marker. Int J Chem Stud 2019;7(3):504-508.
