International Journal of Chemical Studies
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P-ISSN: 2349-8528, E-ISSN: 2321-4902   |   

Vol. 7, Issue 1 (2019)

In-vitro evaluation of Trichoderma spp. for the management of Rhizoctonia oryzae-sativae causing aggregate sheath spot of rice in Manipur


Author(s): Vignesh P, LNK Singh, Bireswar Sinha, Ph Sobita Devi, K Sarda Devi, V Bhuvaneswari and K Dinesh

Abstract: Present research was carried out to understand antagonistic potentiality of six native Trichoderma spp. against Rhizoctonia oryzae-sativae causing Aggregate sheath spot of rice. Dual culture technique revealed the inhibition percentages of R. oryzae-sativae by T. asperellum (KU933475), T. koningiopsis (KU904460), Hypocrea lixii (KX0113223), T. harzianum (KU904458), T. ovalisporum (KU904456) and T. atroviridae (KU933472) of 58.43%, 68.23%, 60.0%, 72.94%, 64.31%, and 70.19% respectively. Bell’s scale study showed class III category by Trichoderma spp and class II showed by T. harzianum (KU904458) against Rhizoctonia oryzae-sativae. Among the different biological agents Trichoderma harzianum is the most effective one for reducing rapid growth of the pathogen. Further, all Trichoderma isolates significantly inhibited the mycelial growth of the pathogen.

Dual culture technique of bio control agents against the growth of R. oryzae-sativae
Fig. 1: Dual culture technique of bio control agents against the growth of R. oryzae-sativae

Percent inhibition of mycelial growth in culture plate
Fig. 2: Percent inhibition of mycelial growth in culture plate

Growth pattern of R. oryzae-sativae and the biological control agents in terms of radius of the colony
Fig. 3: Growth pattern of R. oryzae-sativae and the biological control agents in terms of radius of the colony

Pages: 2498-2501  |  606 Views  109 Downloads

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How to cite this article:
Vignesh P, LNK Singh, Bireswar Sinha, Ph Sobita Devi, K Sarda Devi, V Bhuvaneswari, K Dinesh. In-vitro evaluation of Trichoderma spp. for the management of Rhizoctonia oryzae-sativae causing aggregate sheath spot of rice in Manipur. Int J Chem Stud 2019;7(1):2498-2501.
 

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