Vol. 7, Issue 1 (2019)
Determination of mutagenic sensitivity of chrysanthemum (Dendranthema grandiflora Tzvelev) cv. Pusa Chitraksha to physical and chemical mutagens
Author(s): Rajasekar P, Kannan M and Ganga M
Abstract: An experiment was conducted to determine LD50 does of physical and chemical mutation in cv. Pusa Chitraksha. In a mutation breeding experiment, LD50 gives an indication of the response on different types of explants to mutagen, so that the right dose(s) for the main field experiment can be fixed to obtain beneficial mutants with minimal population loss. In the present investigation, herbaceous cuttings (7 - 10 cm long with 4- 5 nodes) of chrysanthemum cv. Pusa Chitraksha were treated with twenty one different dosages of Gamma rays and EMS ranging from 5.00 to 50.00 Gray and 0.10 to 0.50 per cent respectively. The results revealed a gradual and significant reduction based on survival rates of the treated cuttings using probit analysis as well as the growth rate in different dosage of both mutagens. All the treatments with gamma rays and EMS revealed a dose dependent negative linear relationship between dose and survival of treated cuttings. The survival percentage of variety Pusa Chitrakshain gamma irradiated population ranged from 85.00 per cent in 5 Gy to 10.00 per cent in 45 Gy among the gamma ray treatment. In case of EMS treatments, survival was ranged from 80.00 per cent in 0.10 per cent of EMS and 10.00 per cent in 0.40 per cent of EMS and none of the plants were survived in 0.45 and 0.50 percent ethyl methane sulphonate. Based on the survival of treated cuttings probit curve analysis was exhibited that, LD50 determined as 23.00 Gray for gamma radiation and 0.27 per cent for EMS.
Fig. 1: Probit curve for the determination of LD50 for gamma radiation in Pusa Chitraksha
Fig. 2: Probit curve for the determination of LD50 for gamma radiation in Pusa Chitraksha
Pages: 489-493 | 668 Views 195 Downloads
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How to cite this article:
Rajasekar P, Kannan M, Ganga M. Determination of mutagenic sensitivity of chrysanthemum (Dendranthema grandiflora Tzvelev) cv. Pusa Chitraksha to physical and chemical mutagens. Int J Chem Stud 2019;7(1):489-493.
