Vol. 7, Issue 1 (2019)
Comparative efficacy of RBPT, STAT, Dot-ELISA, Plate ELISA and serum PCR indiagnosis of caprine brucellosis
Author(s): Masood S Mir, Abha Mariam, Shafqat Khan, Vivek Gupta, Omar Khalil Baba, Hilal M Khan, Zahid A Kashoo, Mudasir A Rather, FD Sheikh, Asif H Sofi and Sarfaraz A Wani
Abstract: Present study was aimed at comparing efficacy of serological tests including RBPT, STAT, Dot-ELISA, Plate ELISA and serum PCR in diagnosis of caprine brucellosis using unvaccinated goat population as target. Pair-wise comparison of different tests employed for diagnosis of brucellosis revealed almost perfect agreement was observed between RBPT and Dot-ELISA (k = 0.8366), while substantial agreement was observed between STAT and Plate ELISA (k = 0.7364), Dot ELISA and PCR (k = 0.6414) and Plate ELISA and PCR (k = 0.6642). Results from STAT vs Dot ELISA (k = 0.6009), STAT vs PCR (k = 0.5981), Dot ELISA vs Plate ELISA (k = 0.5572), RBPT vs Plate ELISA (k = 0.5412) and RBPT vs STAT (k = 0.4487) revealed moderate agreement between tests. RBPT and PCR showed a fair agreement with kappa value of 0.3924. Evaluation of relative sensitivity and specificity of different diagnostic tests revealed highest sensitivity (98.78%) for Dot ELISA followed by RBPT (85.3%) while they lack specificity. However specificity of Plate ELISA (99.8%) and PCR (99.4%) was highest but with low sensitivity. The results suggested use of a combination of serological tests for screening, supplemented with direct test like isolation and/or PCR for confirmation and species level identification.
Pages: 308-312 | 576 Views 70 Downloads
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How to cite this article:
Masood S Mir, Abha Mariam, Shafqat Khan, Vivek Gupta, Omar Khalil Baba, Hilal M Khan, Zahid A Kashoo, Mudasir A Rather, FD Sheikh, Asif H Sofi, Sarfaraz A Wani. Comparative efficacy of RBPT, STAT, Dot-ELISA, Plate ELISA and serum PCR indiagnosis of caprine brucellosis. Int J Chem Stud 2019;7(1):308-312.
