Vol. 7, Issue 1 (2019)
Blood biochemical indices lack diagnostic significance in subclinical caprine brucellosis: Evidence from non-vaccinated Brucella melitensis positive Changra goats in Northern Himalayan region of Ladakh
Author(s): MS Mir, Abha Mariam, Shafqat Khan, HM Khan, OK Baba, ZA Kashoo, MA Rather, FD Sheikh, AH Sofi, Asiya Kazmi and Sarfaraz A Wani
Abstract: Caprine brucellosis caused by Brucella melitensis is prevalent globally causing severe economic losses in terms abortions and still births, maintenance of animals with sub-clinical or latent infection and associated labour, besides being an important zoonosis. Cohort serum biochemical profiling aimed at elucidating any possible diagnostic efficacy in caprine brucellosis was undertaken in unvaccinated Pashmina goats. Positive and negative reactors from farmer's flocks were identified by RBPT and STAT, and Brucella melitensis infection confirmed by species specific Dot-ELISA and Plate-ELISA and serum based PCR. Evaluation of serum biochemical parameters with respect to positive vs negative reactor goats revealed non-significant differences in blood glucose (76.697±4.747 vs 67.534±4.019); total protein (5.959±0.106 vs 5.947±0.104); albumin (3.191±0.048; 3.272±0.062); globulin (2.769±0.107 vs 2.675±0.100); AST (62.722±3.839 vs 67.972±4.925); ALT (16.753±1.340 vs 15.684±1.197); ALP (250.670±20.067 vs 266.960±20.100); Creatinine (0.638±0.072 vs 0.713±0.073); urea (30.717±1.443 vs 30.397±1.808); cholesterol (137.214±3.406 vs 138.322±3.363).The serum biochemical indices seemed to be stable with respect to brucellosis at least in sub-clinical or latent phase of infection in goats.
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Fig. 1: Rose Bengal Plate Agglutination test (RBPT): Note the clear clump formation depicting agglutination reaction
Fig. 2: Standard Tube Agglutination Test (STAT):Tubes 1 to 6: dilution1:10, 1:20, 1:40, 1:80, 1:160 & 1:320; Tube C: Control; Note the mat formation upto tube-4 (1:80 dilution), turbidity in tubes 5,6 and control.
Fig. 3: Dot-ELISA for Brucella melitensis: Note blue dot formation in positive samples
Pages: 301-307 | 979 Views 95 Downloads
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How to cite this article:
MS Mir, Abha Mariam, Shafqat Khan, HM Khan, OK Baba, ZA Kashoo, MA Rather, FD Sheikh, AH Sofi, Asiya Kazmi, Sarfaraz A Wani. Blood biochemical indices lack diagnostic significance in subclinical caprine brucellosis: Evidence from non-vaccinated Brucella melitensis positive Changra goats in Northern Himalayan region of Ladakh. Int J Chem Stud 2019;7(1):301-307.
