Vol. 7, Issue 1 (2019)
Effect of in ovo injection of probiotics bacteria Lactobacillus acidophilus on the gut microbial colonization in commercial broilers
Author(s): P Kanagaraju, S Ravichandran, K Kumanan, P Muthusamy, S Rathnapraba and G Srinivasan
Abstract: The respective research trial was conducted to investigate the effect of in ovo injection of Lactobacillus acidophilus to 18 days old broiler chicken embryo on gut microflora of commercial broilers. On 18th day of incubation in ovo injection was carried out in which out of total 720 broiler hatching eggs, 144 eggs served as non injected control (T1), 144 eggs served as sham control and the remaining 432 eggs (144 for each treatment group) were injected with 0.2 ml of 1x106 Lactobacillus acidophilus (T3), 0.2 ml of 1x109 Lactobacillus acidophilus (T4) and 0.2ml of 1x1012 Lactobacillus acidophilus (T5). The positive control group was injected with 0.2 ml of 0.9% normal saline solution. At hatch, 480 chicks were randomly selected (96 birds in each treatment) with six replicates of 16 birds each as per treatment wise. On 42nd day of experiment, data related to gut microflora count of intestinal contents were recorded and statistically analysed. In ovo injection of L. acidophilus how ed significant (P<0.01) improvement in colonization of beneficial Lactobacillus spp. and significant (P<0.01) reduction in harmful bacteria among treatment and control groups. Among the treatment group L. acidophilus of 1x106shows better values of 14.24 compared to other treatment groups with log value of 15.00 in LA 1x109 and 14.94 kg value in LA 1x1012 injected group. The results of the present trial indicated that the in ovo injection of Lactobacillus acidophilus has incresed beneficial bacteria counts and markedly reduced harmful pathogens in the intestinal contents of commercial broilers thereby improved gut efficiency and gut health of broiler chicken.
Pages: 197-200 | 609 Views 65 Downloads
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How to cite this article:
P Kanagaraju, S Ravichandran, K Kumanan, P Muthusamy, S Rathnapraba, G Srinivasan. Effect of in ovo injection of probiotics bacteria Lactobacillus acidophilus on the gut microbial colonization in commercial broilers. Int J Chem Stud 2019;7(1):197-200.
