Vol. 7, Issue 1 (2019)

This investigation concentrated on the isolation and enrichment of cellulolytic microorganisms from residual banana pseudo stem material, capitalizing on its abundant biochemical composition. A total of 69 bacterial varieties were isolated, whereby 20% were successfully enriched utilizing carboxymethylcellulose (CMC) minimal salt medium (MSM), whereas the remaining 80% were acquired through direct cultivation on nutrient agar. Within the isolated strains, 21.25% exhibited active cellulase properties, with strain BSC14 displaying the most extensive zone ratio, followed by strains BSC2 and BSC12. The morphological and cultural analysis disclosed that the majority of cellulolytic isolates were Gram-positive and rod-shaped, showcasing a variety of colony sizes and forms. These outcomes highlight the potential of banana pseudo stem residue as a viable source for isolating cellulolytic bacteria, presenting encouraging prospects in the realms of industrial biotechnology and sustainable waste management.