Vol. 6, Issue 6 (2018)
An in silico approach for evaluation of rbcL and matK loci for DNA barcoding of fabaceae family
Author(s): Sumana Sikdar, Sharad Tiwari, Vishwa Vijay Thakur and Swapnil Sapre
Abstract: The main aim of present work is to demonstrate effectiveness of in silico approach to gain a prior insight into the efficacy of the rbcL and matK loci as DNA barcodes for a group of plants belonging to fabaceae family. For this in silico analysis, we retrieved all available sequences of selected plants species from NCBI and examined for their species resolution ability. The species resolution ability of matK and rbcL loci was varied from genus to genus. In genus Vigna, Cassia and Crotolaria, matK is an ideal locus for DNA barcoding whilst in genus Sesbania, rbcL has proved their efficacy. This in silico approach makes the process of validation of novel barcodes much easier by decreasing the number of candidate primer pairs which are required to be tested in vitro.
Pages: 2446-2451 | 683 Views 150 Downloads
download (8748KB)
How to cite this article:
Sumana Sikdar, Sharad Tiwari, Vishwa Vijay Thakur, Swapnil Sapre. An in silico approach for evaluation of rbcL and matK loci for DNA barcoding of fabaceae family. Int J Chem Stud 2018;6(6):2446-2451.
