Vol. 6, Issue 6 (2018)
Assessment of biochemical yield variation in small millets germplasm lines
Author(s): Sanjeev Kumar Yadav, AK Sharma, Sushma Tiwari and Pramod Kumar Yadav
Abstract: Small millets are the group of six crops comprising of finger millet, kodo millet, little millet, foxtail millet, barnyard millet and proso millet. These are considered as nutri-cereals and are source of food, feed and fodder. The crops were grown in a variety of agro-ecological situations including plains, coast and hills as well as in diverse soils and varying rainfall. Three general types of methods have been developed for isolating and analyzing dietary content of fibers, carbohydrates, phenol and protein in small millets i.e: enzymatic-gravimetric, DNS acid, Folin-Ciocalteu's assay method and Lowrys method respectively. Protein was estimate according to the procedure of Lowry et al., (1951). The protein in the crude extracts was estimated after precipitation with an equal volume of 10% (w/v) trichloro acetic acid. The mixture was incubated for 30 min at -20 degree celsius and then centrifuged at 10,000g for 10 min. The precipitate was dissolved in 0.5 ml of 1 N NaOH. A standard curve for the protein estimation was prepared by using bovine serum albumin fraction V. The absorbance of the protein sample was measured at 660 nm after 30 min incubation with the reagent mixture.3,5-Dinitrosalicylic acid can be prepared by the nitration of salicyclic acid they reacts with reducing sugars and other reducing molecules to form 3-amino-5-nitrosalicylic acid, which strongly absorbs light at 540 nm. The original AOAC enzymatic-gravimetric method for the determination of total dietary fibre (TDF) was developed on basis of the joint experience.
Pages: 1809-1815 | 627 Views 209 Downloads
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How to cite this article:
Sanjeev Kumar Yadav, AK Sharma, Sushma Tiwari, Pramod Kumar Yadav. Assessment of biochemical yield variation in small millets germplasm lines. Int J Chem Stud 2018;6(6):1809-1815.
