Vol. 6, Issue 6 (2018)
Screening and validation of known DNA markers for BPH resistance in Germplasm of rice (Oryza sativa L.)
Author(s): Prakriti Meshram, Sandeep Bhandarkar, Shubha Banerjee, SK Nair, DK Rana and AK Sarawgi
Abstract: Rice productivity is adversely impacted by numerous biotic and abiotic factors. Diseases and insect pests are the major biotic agents causing significant yield losses. DNA marker based technology is being increasingly used to overcome difficulties of plant breeding based on phenotypic characters like insect resistance. The experimental material consisted of 24 elite rice genotypes was involved, where T- 12 and ARC 10550 were used as resistant check and Swarna used as susceptible check, respectively. Total rice genomic DNA was extracted from young succulent, disease and insect free seedlings by mini prep method. The DNA was extracted from 24 lines out of which 13 were resistant and 11 were susceptible.PIC value ranges from B (monomorphic) to A (very high discriminative with many alleles in equal frequencies). The average PIC value for all 10 microsatellite loci in the present study was 0.60, with a range from 0.329- 0.818. PIC was highest for the SSR primer RM5479 (0.818), and was lowest for the primer RM589, (0.329). Hence, primer RM 5479 is highly informative in the present study. The association between trait and markers were estimated using single marker analysis (SMA) in Microsoft Excel program. The significant marker trait associations were indicated by a P-value (< 0.05) along with corresponding R2 for each marker is the total phenotypic variation for a trait that is accounted by markers. We detected a significant resistance marker-trait association (P<0.05). All of the significant SSR loci were indentified for the agronomic traits, with the R2, percentage of the total variation explained ranging from 9.73% to 35.5%. In the analysis of susceptibility/resistance index marker RM6869 show the resistance, it shows significant (at 5%) P-value 0.041% and %R 2 is highest 35.5% that indicates the Marker RM6869 is associated with gene Bph2 and Bph18 genes present in BPH resistance germplasm.
Pages: 1540-1542 | 347 Views 68 Downloads
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How to cite this article:
Prakriti Meshram, Sandeep Bhandarkar, Shubha Banerjee, SK Nair, DK Rana, AK Sarawgi. Screening and validation of known DNA markers for BPH resistance in Germplasm of rice (Oryza sativa L.). Int J Chem Stud 2018;6(6):1540-1542.
