Vol. 6, Issue 6 (2018)
Preparation and properties of lantadene a complex with DNA
Author(s): Arti Ghabru, RG Sud and OP Sharma
Abstract: Lantana (Lantana camara L.) is a hepatotoxic plant with a number of medicinal properties. Lantadene A (LA), a pentacyclic triterpenoid present in the leaves of lantana plant is one of the most important bioactive constituents of this plant. Pentacyclic triterpenoids exhibit a number of bioactivities like antitumor, anti-HIV, anti-inflammatory, antitubercular, inhibition of nitric oxide production, inhibition of DNA polymerase and DNA topoisomerase. LA was isolated from lantana leaves and purified by batch processes, column chromatography and fractional crystallization. The purity as ascertained by HPLC analysis. The chemical structure was confirmed as 22β-angeloyloxy-3-oxoolean-12-en-28-oic acid by spectroscopic analysis. LA-DNA interaction was ascertained y electrophoresis. The observations on interaction of LA with DNA as evaluated by agrose gel electrophoresis were very interesting. LA masked the binding of ethidium bromide to DNA implying thereby that lantadene. An interacted with DNA by intercalation. Future studies are required to use the LA-BSA for development of immunoassay and for development of possible hepatoprotective immunogen against lantana toxicity. The observation on the intercalation of LA with DNA implies that LA could be potential antitumor compound for future drug development.
Pages: 1511-1515 | 398 Views 75 Downloads
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How to cite this article:
Arti Ghabru, RG Sud, OP Sharma. Preparation and properties of lantadene a complex with DNA. Int J Chem Stud 2018;6(6):1511-1515.
