Vol. 6, Issue 6 (2018)
In vitro regeneration and rapid multiplication of Dendrobium nobile
Author(s): Priyanka S, Verma LS, Satyanarayana E and Subhankar
Abstract: An experiment was conducted on in vitro regeneration and multiplication of Dendrobium sp. Different concentrations of NAA and BAP alone or combination of both hormones were used as treatment for regeneration. It was revealed that shoot regeneration from node was the best at 2.5 mg/l BAP supplemented to MS medium. It gave better responses than all other concentrations and combinations of NAA and BAP, used in the present study. The highest number of shoots and leaves were found when 0.5 mg/l NAA with 1.0 mg/l BAP was supplemented into MS medium. For rooting, 1.0 mg/l IBA with activated charcoal was found to be the most effective. The well-rooted plantlets were successfully acclimatized under 70-80% humidity and planted in pots and transferred to the shade house for establishment. Around 85% of plantlets survived in the field.
Pages: 1286-1288 | 619 Views 204 Downloads
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How to cite this article:
Priyanka S, Verma LS, Satyanarayana E, Subhankar. In vitro regeneration and rapid multiplication of Dendrobium nobile. Int J Chem Stud 2018;6(6):1286-1288.
