Vol. 6, Issue 6 (2018)
Studies on in vitro regeneration of orchids (Dendrobium nobile) using shoot explant
Author(s): Priyanka S, Verma LS, Satyanarayana E and Subhankar
Abstract: Shoot tips of Dendrobium sp. were used as explants and inoculated on MS medium at different concentrations. The maximum callus formation (83%) and the maximum number of callus initiated (12.00) were observed in combination of NAA and BAP at 1mgL-1 into MS medium after 80 days of culture. Subcultured callus were inoculated on MS medium supplemented with different combinations of NAA (0.5, 1.0, 1.5, 2.0 mgL-1) and BAP (0.5, 1.0, 1.5, 2.0 mgL-1) for shoot regeneration. The maximum number of shoot (12.00), the highest shoot length (3.613 cm) and the plantlet regeneration were observed in 0.5 mgL-1 NAA + 1.0 mgL-1 BAP after 60 days of culture. Even, the maximum number of root (4.00), the maximum root length (1.627cm) and the maximum regeneration percentage (91.44%) were observed in 0.5 mgL-1 NAA + 1.0 mgL-1 BAP after 60 days of culture. Finally, regenerated plantlets were transferred into half strength MS medium to obtain plants.
Pages: 1283-1285 | 569 Views 133 Downloads
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How to cite this article:
Priyanka S, Verma LS, Satyanarayana E, Subhankar. Studies on in vitro regeneration of orchids (Dendrobium nobile) using shoot explant. Int J Chem Stud 2018;6(6):1283-1285.
