Vol. 6, Issue 6 (2018)
Genetic diversity analysis of teak in South Gujarat by RAPD marker
Author(s): Chintankumar Chaudhari1, Suman Kumar Jha, Ravindra Kumar Dhaka, Vipulkumar Parekh, MS Sankanur, Pravin Prajapat and Shikha Thakur
Abstract: Teak is a deciduous tree species belonging to the Lamiaceae family producing good quality timber. Its wood is rightly famous for furniture making due to fine grain, mellow color and durability. The present investigation was carried out to assess the genetic diversity in teak in south Gujarat by RAPD molecular marker. Out of 30 RAPD primers tested, 20 primers produced clear and reproducible band. The number of loci amplified by these RAPD primers ranged between 4 (OPD-02 and OPF-09) to 15 (OPF-01). A total of 192 loci were amplified out of which 150 (78.13%) loci were polymorphic. The percentage of polymorphism ranged from 25% for OPD-02 to 100% for OPF-06. Highest PIC value (0.5), effective multiplex ratio (6.34), Marker Index (2.99) and resolving power (8.60) observed with OPD-03, OPF-05, OPD-03 and OPF-01, respectively. Maximum Jacard’s similarity coefficient (0.81) and Nei& Li’s coefficient (0.89) observed between Vansada and Dharampur, indicating a close relationship between these populations. However, the smallest Jacard’s similarity value (0.47) and Nei& Li’s coefficient (0.64) were observed between Vyara and Bardipada-B. Dendrograms of the accessions were constructed based on the Jacard’s coefficient and Nei& Li’s coefficient gave exact similar clustering of the ten populations. All the populations from Valsad Forest division tend to fall in same cluster. Populations from Dang forest division stayed in same cluster except Bardipada-B population. The PCoA results corresponded well with the cluster analysis obtained through UPGMA. The first three coordinates accounted for 20.53, 14.90 and 13.31 percent of the total variance, respectively. Thus, the total cumulative variance accounted by these three coordinates was 48.73 percent. Overall, the Vyara population was quite distinct.
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How to cite this article:
Chintankumar Chaudhari1, Suman Kumar Jha, Ravindra Kumar Dhaka, Vipulkumar Parekh, MS Sankanur, Pravin Prajapat, Shikha Thakur. Genetic diversity analysis of teak in South Gujarat by RAPD marker. Int J Chem Stud 2018;6(6):260-267.
