Vol. 6, Issue 5 (2018)
Molecular variability in isolates of Rhizoctonia bataticola causing root rot in chickpea by issr marker
Author(s): Puja Walunj, SS Mane, Renuka Tatte, Priyanka Lohar and Dhanashri Dhepe
Abstract: The Genetic diversity of all the isolates of Rhizoctonia bataticola was analysed by using Inter-Simple Sequence Repeat (ISSR) markers. The ISSR analysis, a total 10 primers were screened against eight isolates of Rhizoctonia bataticola. Out of 10 primers 9 primers amplified scorable banding pattern. Out of 37 bands, 30 bands were polymorphic and 7 bands are monomorphic. Average level of Polymorphism was 81.08%. In the dendrogram, Rb-2 (Nagpur) was found to have higher value of similarity coefficient (0.808) whereas Rb-1(Akola) was found to have lower value of similarity coefficient (0.375). The isolates of Rhizoctonia bataticola were grouped into four major clusters. First group is named as cluster-A, includes isolates belonging to Akola, Amravati and Sangli. Second group is named as cluster-B, includes Nagpur, Karanje and Kini, third group is named as cluster-C, includes Umbraj and fourth grouped is named as cluster-D includes Kurundwad. It shows that Rb-2 (Nagpur) have higher value of similarity coefficient with Rb-5 (Karanje) whereas Rb-1 (Akola) have lower value of similarity coefficient with Rb-8 (Kini). The similarity matrix indicated that eight isolates of Rhizoctonia bataticola exhibited (37.5 to 80.8) percent similarity coefficient.
Pages: 3374-3378 | 573 Views 161 Downloads
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How to cite this article:
Puja Walunj, SS Mane, Renuka Tatte, Priyanka Lohar, Dhanashri Dhepe. Molecular variability in isolates of Rhizoctonia bataticola causing root rot in chickpea by issr marker. Int J Chem Stud 2018;6(5):3374-3378.
