Vol. 6, Issue 5 (2018)
Molecular characterization of Colletotrichum capsici causing anthracnose of chilli
Author(s): Dhanashri Dhepe, Ekta Bagde, Renuka Tatte, SS Mane and Puja Walunj
Abstract: The Random Amplified Polymorphic DNA (RAPD) technique was employed to detect the genitical variation among the four isolates of Colletotrichum capsici. Out of 20 randomly selected RAPD primers screened, 16 primers produced 117 scorable bands. Among the RAPD primer 117bands 100 bands were polymorphic and level of polymorphism was 85.47%. The result from the UPGMA analysis based dendrogram generated for Colletotrichum capsici isolated revealed that they were divided into two main clusters, cluster I and II. The highest genetic similarity to an extent of 49.50% was recorded between Cc1 and Cc2 isolates and the least genetic similarity that is 29.30% noticed between Cc2 and Cc3.Cc3 is distinct and showed least similarity with all other isolates of Colletotrichum capsici. Cc3 may be present in temprate region (Western Himalayan Region).
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How to cite this article:
Dhanashri Dhepe, Ekta Bagde, Renuka Tatte, SS Mane, Puja Walunj. Molecular characterization of Colletotrichum capsici causing anthracnose of chilli. Int J Chem Stud 2018;6(5):853-856.
