Vol. 6, Issue 4 (2018)
Morphological and molecular diversity study among the isolates of Trichoderma using RAPD markers
Author(s): Sonia Kaushal, Sunita Chandel, Monica Sharma and Priyanka Kashyap
Abstract: Species of Trichoderma are most commonly exploited fungus as a biological control agent and the present study involves the diversity study on molecular basis. Morphological methods have been diminished due to the use of molecular techniques which have made advances in systematic characterization of Trichoderma spp. Twelve different random primers viz., OPA-01, OPA02, OPA03, OPA-04, OPA-05, OPA06, OPA07, OPA08, OPA09, OPA-10, OPA12 and OPA-13 were screened for generating polymorphism among the randomly selected isolates under the study out of which three primers namely OPA04, OPA05 and OPA10 were selected to amplify with all the seven isolates. Trichoderma isolates differed in their morphological behavior while on molecular basis, maximum polymorphism was observed in the randomly selected isolates. All the bands observed were polymorphic in nature indicating the genetic diversity among Trichoderma spp.
Pages: 1731-1735 | 386 Views 53 Downloads
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How to cite this article:
Sonia Kaushal, Sunita Chandel, Monica Sharma, Priyanka Kashyap. Morphological and molecular diversity study among the isolates of Trichoderma using RAPD markers. Int J Chem Stud 2018;6(4):1731-1735.
