Vol. 6, Issue 4 (2018)
A simple method for quantitative determination of venlafaxine in blood by high-performance liquid chromatography
Author(s): Georgi Bonchev, Snezha Zlateva, Petko Marinov, Ivelina Stefanova and Ivaylo Vazharov
Abstract: Although venlafaxine is generally considered as a relatively safe antidepressant, in unfavorable circumstances it could lead to unexpectedly severe intoxications. Examples are intentional over dosage cases, especially when combined with other psychotropic medications. That is why the main purpose of this work was to develop and optimize an express yet precise method for blood venlafaxine determination that will be a valuable asset to analytical toxicology lab at any emergency toxicology department. The developed method uses RP-HPLC on C18 column (150×4.6 mm, 5 μm) under isocratic conditions at 25 °C. As a mobile phase a 22:78 v/vacetonitrile:phosphate buffer (pH 5.2) mixture at flow rate 1.0 mL/min has been chosen.Fluorescent detection (ex. 228 nm; em. 300 nm) has proven to give best results. Validation of the method in human plasma has shown an 86% yield, a good linearity (Pearson's r > 0.9997)over a wide concentration range (0.01 – 10.0 µg mL–1), and an excellent precision (RSD 0.60%). LOQ for venlafaxine has been estimated to 10ng mL–1. The method has been used for treatment monitoring in a clinical case of combined acute intoxication (venlafaxine and valproic acid). Severance of manifested toxic symptoms, not in correspondence to medications' blood levels, has been pointed out. Drug interaction has been assumed as a possible explanation for mutual toxicity escalation.
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How to cite this article:
Georgi Bonchev, Snezha Zlateva, Petko Marinov, Ivelina Stefanova, Ivaylo Vazharov. A simple method for quantitative determination of venlafaxine in blood by high-performance liquid chromatography. Int J Chem Stud 2018;6(4):801-804.
