Vol. 6, Issue 4 (2018)
Stability of calcium phosphate nanoparticle adjuvanted outer membrane protein in term of zetapotential
Author(s): Mahmoodullah, S Tamuly and S Sarma
Abstract: The nanoparticles are widely used in various areas such as drug delivery, vaccine adjuvants, as an antibacterial and so on. The property of these nanoparticles is retained only if they are stable in aqueous solution. The nanoparticles have high surface energy given to their large surface to volume ratio that generally makes them highly unstable and have the tendency to aggregate. There are various methods by which, the aggregation can be prevented such as altering ionic environment, pH or incorporation of protein. The stability can be assessed by measuring the zeta potential. In this study, we have determined the zeta-potential of the Salmonella Typhimurium outer membrane protein (OMP) entrapped in calcium phosphate nanoparticles (CAP-NP) at pH 6.0, 6.5, 7.0 and 7.5. It was observed that there was no significant difference among the zeta-potential of calcium phosphate nanoparticle-OMP complex suspended in PBS of pH 6.0, 7.0 and 7.5. However, the zeta-potential at pH 6.5 was significantly lower. The vaccine was found to be stable at pH 7.0 to 7.5 and highly unstable at pH 6.5 in term of zetapotential.
Pages: 680-684 | 643 Views 105 Downloads
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How to cite this article:
Mahmoodullah, S Tamuly, S Sarma. Stability of calcium phosphate nanoparticle adjuvanted outer membrane protein in term of zetapotential. Int J Chem Stud 2018;6(4):680-684.
