Vol. 6, Issue 4 (2018)
Preparation of Porcine Acellular Diaphragm Matrix (ADM) using sodium deoxycholate as a biological detergent for application in surgical reconstruction
Author(s): Parsha Jyoti Nath, Kushal Konwar Sarma, Bhupen Sarma, Satya Sarma, Tirtha Nath Upadyayaya, Dhireswar Kalita and Biswajit Dutta
Abstract: Acellular Tissue Matrix is now been considered suitable alternatives to synthetic materials in reconstructive surgery. In the present study, fresh porcine diaphragm was subjected to biological detergent treatment under physical agitation in orbital shaker to make it cell free. To standardize the decellularization protocol, different concentrations of Sodium Deoxycholate (1%, 1.5%, 2% and 2.5%) solutions were used and the cut pieces of diaphragm were kept under physical agitation in an orbital shaker (180 rpm) at 35 0C till 36 h. The detergent solution in each concentration was changed in every 6 h and representative samples were kept in 10 % neutral formalin in every 12 h. The histological analysis from each representative tissue sample with different concentration and duration combinations were carried out to standardize the optimum decellularization protocol. Fresh Porcine Diaphragm treated with 2% sodium deoxycholate under physical agitation in orbital shaker at 35 0C for 24 h was found to be efficient enough for complete removal of cells as well as maintaining the normal collagen fibre pattern when compare to other combinations.
Pages: 301-303 | 734 Views 65 Downloads
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How to cite this article:
Parsha Jyoti Nath, Kushal Konwar Sarma, Bhupen Sarma, Satya Sarma, Tirtha Nath Upadyayaya, Dhireswar Kalita, Biswajit Dutta. Preparation of Porcine Acellular Diaphragm Matrix (ADM) using sodium deoxycholate as a biological detergent for application in surgical reconstruction. Int J Chem Stud 2018;6(4):301-303.
