Vol. 6, Issue 4 (2018)
Development of spectroscopic method for quantification of sodium hydroxide in milk
Author(s): Modi Zeel S, Desai Bhoomikaben S, SC Parmar, AK Jain, AI Shaikh and KD Aparnathi
Abstract: A spectroscopic technique was utilized to develop method for quantification of sodium hydroxide in milk. Colour was developed by reaction between sodium hydroxide and rosolic acid and absorption was measured at 554 nm. Standard curve was constructed for concentration of sodium hydroxide versus absorbance. Recovery factor was developed from amount of spiked sodium hydroxide and amount of sodium hydroxide obtained from standard curve. After developing the method, it was validated for performance by true (known) sample approach. The concentration of sodium hydroxide obtained by application of the developed method was compared with the concentration of the sodium hydroxide actually added for preparation of the sample. For statistical analysis t-test: two sample assuming equal variances was applied. The validation of method developed for quantification of sodium hydroxide suggested that the difference between the estimated amount of sodium hydroxide and amount of sodium hydroxide actually added to milk was statistically non-significant.
Pages: 44-48 | 1602 Views 478 Downloads
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How to cite this article:
Modi Zeel S, Desai Bhoomikaben S, SC Parmar, AK Jain, AI Shaikh, KD Aparnathi. Development of spectroscopic method for quantification of sodium hydroxide in milk. Int J Chem Stud 2018;6(4):44-48.