Vol. 6, Issue 3 (2018)
Testing the effect of silver nanoparticles on the radial growth of R. Solani, Trichoderma Spp. and Pseudomonas fluorescens in vitro
Author(s): N Chiranjeevi, P Anil kumar, R Sarada jayalakshmi, KV Hari Prasad and TNVKV Prasad
Abstract: In the present investigation, efforts were made to test the effect of nano preparations on the growth of R. Solani, Trichoderma Spp. and Pseudomonas flourescens using poisoned food technique. nano preparations from R. solani, Trichoderma isolate ET-1 five day old culture filtrates, P. fluorescens isolate PF-2 five day old culture filtrate and nano preparations from PDB were assessed along with pure silver nitrate solution at 50%, 5%, 0.5%, 0.05%, 0.005% concentrations of 170 ppm silver nitrate or silver nano preparation. Observations on radial growth were recorded at on 1, 2, and 3 days after inoculation. Studies using poisoned food technique indicated that silver nitrate preparation (pure or bionano) had fungistatic effect on R. solani at lower concentrations (5% and below) as the inhibitory effect was nullified after three days of inoculation. However, at higher concentrations, i. e., at 50% concentration, inhibitory effect continued to exist even after third day. Further, it is also interesting to note that the silver nano preparation from R. solani was inhibitory to itself. The fungistatic effect of silver preparations at <5% conc. were also observed on Trichoderma ET-1 when assessed using poisoned food technique. Similar to R. solani, Trichoderma ET-1 was also more sensitive to silver preparations at 50% concentration. The inhibitory effect of silver preparations was found more of bactericidal in nature when the sensitivity of P. fluorescens PF-2 was assessed using inhibition zone technique as evidenced by continued inhibition zone even after 4 days of seeding the agar.
Pages: 1917-1922 | 443 Views 58 Downloads
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How to cite this article:
N Chiranjeevi, P Anil kumar, R Sarada jayalakshmi, KV Hari Prasad, TNVKV Prasad. Testing the effect of silver nanoparticles on the radial growth of R. Solani, Trichoderma Spp. and Pseudomonas fluorescens in vitro. Int J Chem Stud 2018;6(3):1917-1922.
