Vol. 6, Issue 3 (2018)
HPLC-MS and HPTLC Finger printing of fractions of Asteracantha longifolia Leaf extract
Author(s): Amit K Dash, GK Dutta, G Sahoo, A Maity, KK Sardar and MK Panda
Abstract: Chromatographic techniques can be used to investigate the molecular fingerprinting of the herbal compounds. In the present study, we explore the HPLC-MS and HPTLC finger printing pattern of three fractions (methanol soluble, water soluble, and residual fraction) of hot water leaf extract of Astercantha longifolia (AL) Linn. Nees. Methanolic and residual fractions were fractionated into two peaks each by HPLC with retention times of 1.07, 4.72 min and 0.95, 1.15 min, respectively. On the contrary, only one peak was observed in the aqueous fraction with a retention time of 0.91 min using a Nova- Pak ® C-18 column. Considering the m/z values it could be attributed that methanolic and aqueous fraction may contain a relatively high percentage of a compound having m/z value of 118. Methanolic and residual fraction has an unique m/z value of 114 which was absent in the aqueous fraction. Methanolic fraction has distinct m/z values of 132, 142 and 261 which makes it separate from the other two fractions. Thus, methanolic fraction contains the more number of compounds in comparison to other two fractions. Scanning of HPTLC developed plates of different fractions at 254 nm showed the presence of nine, six and five peaks in methanolic, aqueous and residual fractions, respectively. However, scanning at 366 nm showed the presence of only three, four and two peaks respectively in these fractions. Thus, 254 nm was found to be optimal wavelength for identification of more compounds compared to 366 nm.
Pages: 1767-1774 | 597 Views 122 Downloads
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How to cite this article:
Amit K Dash, GK Dutta, G Sahoo, A Maity, KK Sardar, MK Panda. HPLC-MS and HPTLC Finger printing of fractions of Asteracantha longifolia Leaf extract. Int J Chem Stud 2018;6(3):1767-1774.
