Vol. 6, Issue 3 (2018)
Effect of seed treatment and spray with biosilver nano particles and bio control agents on defence related enzymes activity in rice seedlings and plant growth parameters in vitro
Author(s): N Chiranjeevi, P Anil Kumar, R Sarada Jayalakshmi, KV Hari Prasad and TNVKV Prasad
Abstract: In the present study Silver nano particles were synthesized using two potential isolates of Trichoderma, Psudomonas fluorescens and one isolate of pathogen Rhizoctonia solani cell free culture filtrates incubated at different days 0, 5, 10 and 15. These synthesized nano particles were characterized by using Zeta potential, Dynamic light scattering (DLS) and Scanning electron microscopy. Four different defence related enzymes, viz., Peroxidise, Poly Phenol Oxidase (PPO), Phenyl Ammonia Lyase (PAL), Tyrosinase were assessed from rice seedlings obtained from two nano treatments, two biocontrol agents and carbendazim applied either as seed treatment or spray. Experiment was conducted in pot culture. Observations were recorded on tenth and fifteenth day for seed treatment and after 24 h and 48 h for spray treatments. Enzymes analysis was done for leaves and roots separately. When the rice seedlings, from either 10% bionano treated seeds or from foliar sprayed plants, were assessed for the accumulation of plant defense related enzymes viz., peroxidases, polyphenol oxidase, phenyl alanine ammonia lyase and tyrosinase, substantial accumulation was observed as evidenced by the absorbance values indicating induction of resistance mechanism in rice seedlings. The present investigation also revealed increased germination of rice seeds, shoot length, root length, S:R ratio, fresh weight, dry weight and vigour index of rice seedlings These nano preparations alone could increase the growth parameters either on par with respective biocontrol agents or sometimes even better when the rice seeds of cv. NLR 34449 were treated with nano preparations and sown in pathogen uninoculated pots indicating their utility in increasing plant growth in place of even biocontrol agents and fungicide seed treatment.
Pages: 882-890 | 446 Views 31 Downloads
How to cite this article:
N Chiranjeevi, P Anil Kumar, R Sarada Jayalakshmi, KV Hari Prasad, TNVKV Prasad. Effect of seed treatment and spray with biosilver nano particles and bio control agents on defence related enzymes activity in rice seedlings and plant growth parameters in vitro. Int J Chem Stud 2018;6(3):882-890.