International Journal of Chemical Studies
Vol. 6, Issue 3 (2018)
Optimization of protocol for large-scale propagation of cv. Patakapura: promising local cultivar of banana plant of Odisha
Author(s): Vijya Laxmi and Souvagyalaxmi Sahoo
Abstract: Banana cv. Patakapura (musa spp.), local variety of Odisha have large commercial value. The present investigation deals with development of an efficient micro propagation protocol for banana cv. Patakapura using shoot tip from young suckers as explant. Types of cytokinin and auxin and their concentration significantly influenced the initial growth, multiplication, shooting and rooting of banana plants in vitro. Maximum callus induction of banana cv. Patakapura was observed on MS medium supplemented with BAP (8.0 mg/l) in combination with NAA (2.0 mg/l). Callus were multiply strongly on MS medium supplemented with BAP (6.5 mg/l) in combination with NAA (1.6 mg/l). Additionally, maximum shoot proliferation and elongation were observed with BAP (5.5 mg/l) and NAA (1.2 mg/l) and better rooting response was observed in NAA (4.0 mg/l) with activated charcoal. Thus, we observed that MS medium in combination with BAP and NAA growth regulators is best for banana cv. Patakapura. After hardening and acclimatization the plantlets were transferred to the field and showed ~75-85 % survival.
Pages: 230-233 | 504 Views 11 Downloads
How to cite this article:
Vijya Laxmi, Souvagyalaxmi Sahoo. Optimization of protocol for large-scale propagation of cv. Patakapura: promising local cultivar of banana plant of Odisha. Int J Chem Stud 2018;6(3):230-233.