International Journal of Chemical Studies
Vol. 6, Issue 2 (2018)
Molecular differentiation and classification in relation to fragrance of landraces and improved varieties of aromatic rice using microsatellite markers
Author(s): Manisha Priyadarshini, Pankaj Kumar and VK Sharma
Abstract: A study was conducted to examine the nature and extent of genetic differentiation and divergence in relation to fragrance among eighteen landraces and improved varieties of aromatic rice using a specific set of eighteen microsatellite markers. Altogether 123 allelic variants including 76 shared and 47 unique alleles were detected with an average of 6.8 alleles per primer, revealing genetic polymorphism due to differences in overall size of amplified products. Appearance of more than one band in some of the entries was observed in combinations with the primers RM 223, RM 252, RM 444, RM 505, RM 252, RM 7049 and RM 7356. Considering the magnitude of polymorphic information content, number of alleles, polymorphism per cent and greater efficiency to unambiguously discriminate the entries, seven primers, namely, RM 256, RM 284, Aro7, RM 223, RM 252, RM 444 and RM 7356, appeared to be highly informative. Occurrence of null allele with primer pair RM 505 was noticed in one of the entries. Microsatellites with CT, GA and AT di-nucleotide motifs detected comparatively greater number of allelic variants. Ample genetic variation amongst the entries was inferred on the basis of similarity coefficients. Basically, the entries were divided into three groups, which were further divided into clusters and sub-clusters. Highly aromatic landraces and improved varieties were accommodated separately into two different groups and third group contained a combination of both types. Principal coordinate analysis completely supported the agglomerative hierarchical classification and neighbor joining tree based grouping of entries. Molecular analysis revealed unique or entry specific alleles which could be useful as molecular fingerprints. Conclusively, molecular profiling exhibited higher level of genetic polymorphism, allowing unique genotyping and unambiguous discrimination of traditional and improved varieties of aromatic rice.
Pages: 3014-3021 | 227 Views 13 Downloads
How to cite this article:
Manisha Priyadarshini, Pankaj Kumar, VK Sharma. Molecular differentiation and classification in relation to fragrance of landraces and improved varieties of aromatic rice using microsatellite markers. Int J Chem Stud 2018;6(2):3014-3021.