International Journal of Chemical Studies
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P-ISSN: 2349-8528, E-ISSN: 2321-4902   |   Impact Factor: GIF: 0.565 NAAS Rating: 5.31 NEW

International Journal of Chemical Studies

Vol. 6, Issue 2 (2018)
16S rRNA gene sequencing for bacterial identification of pullulanase synthesizing thermophilic bacteria contributing to big data


Author(s): Anshul Sharma Manjul and Poonam Shirkot

Abstract: 16S rRNA gene sequences has been by far the most common housekeeping genetic marker used to study bacterial phylogeny and taxonomy because 16S rRNA gene is present in almost all bacteria and its functioning has not changed over time. Present experiment aimed isolation and characterization of thermostable pullulanase producing bacteria. Characterization was done by morphological and biochemical test and was confirmed by molecular approach using 16S rRNA gene sequencing technology. 16S rRNA gene sequence was analyzed with various bacterial genomes using BLASTn tool in NCBI (https://www.ncbi.nlm.nih.gov/) sequence database. Phylogenetic relationships and DNA sequencing showed that the bacterial isolate has 96% similarity with Bascillus licheniformis strain BCRC 11702. Phylogenetic analysis and pairwise alignment revealed its close synteny with related species. A total of 100 bootstrap replications were performed to represent the evolutionary history of the taxa analysed. 16S rRNA gene sequencing has been used to characterize numerous bacterial genera and species. Discovery, phylogenetic relationships and the classification of novel bacterial species has been facilitated by using 16S rRNA gene sequencing. Further, in last decade sequencing of various bacterial genomes and comparison between genomes has confirmed the representativeness of the 16S rRNA gene in bacterial phylogeny. Characterization of thermostable pullulanase synthesizing bacteria would be helpful in defining wide variety of applications in near future as thermostable pullulanase enzyme has many applications in textiles, detergents, food, pharmaceuticals, leather, bioremediation of waste material, starch processing industry and it has a great potential to convert agro-waste into biofuel as it ferments starch to ethanol etc.

Pages: 2769-2773  |  370 Views  5 Downloads

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How to cite this article:
Anshul Sharma Manjul, Poonam Shirkot. 16S rRNA gene sequencing for bacterial identification of pullulanase synthesizing thermophilic bacteria contributing to big data. Int J Chem Stud 2018;6(2):2769-2773.
International Journal of Chemical Studies