Vol. 6, Issue 2 (2018)
Isolation and characterization of eco-friendly microflora for degradation of herbicide and promoting the soil health
Author(s): Uma, Tapas Chowdhury, SB Gupta, Dhanni Devi and Ashok Kumar Koshariya
Abstract: The investigation was carried out in an Inceptisol with Rabi season chickpea to evaluate the isolation of eco-friendly microflora for degradation of herbicide and promoting the soil health. Fifteen soil samples were collected from different plots of a long term herbicidal trail where different herbicides were applied in kharif and rabi season continuously for last five years in a rice-chickpea cropping system. From these soil samples 11 crop beneficial herbicide tolerant microbial isolates were collected. These isolates were further characterized with respect to their cultural characteristics, Dehydrogenase activity and these selected isolates were further tested for their potentiality of pendimethalin degradation. Three rhizobial and four phosphobacterial isolates were taken in a study for their herbicide degradation potential under in-vitro conditions. All isolates had shown good growth in their respective culture media containing 5000 ppm pendimethalin. Twenty treatments were taken under this study comprising single and dual inoculation of above symbiotic N-fixing and P-solubilizing microorganisms, including control. All the treatments were compared with uninoculated control. Treatment No. 19 dual inoculation of rhizobial isolate Rhizobium-3 and phosphobacterial isolate PSB-4 was found to be best for rapid degradation of herbicide pendimethalin, followed by single inoculation of phosphobacterial isolate PSB-4. These native isolates had soon highest Dehydrogenase activity over control at 50 DAS. The combined application of Rhizobium-3 and PSB-4 was also found supreme to increase the chickpea yield at highest level followed by isolate PSB-4 by mobilizing more nitrogen and phosphorus in crop rhizosphere.
Pages: 1970-1974 | 605 Views 45 Downloads
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How to cite this article:
Uma, Tapas Chowdhury, SB Gupta, Dhanni Devi, Ashok Kumar Koshariya. Isolation and characterization of eco-friendly microflora for degradation of herbicide and promoting the soil health. Int J Chem Stud 2018;6(2):1970-1974.
