International Journal of Chemical Studies
Vol. 6, Issue 2 (2018)
Effect of cold pre-treatment on callus induction and shoots regeneration in ovary culture of marigold (Tagetes spp)
Author(s): Thaneshwari, Aswath C and Chaithra N
Abstract: Marigold (Tagetes spp) is recognized to have potential usage in medicinal and pharmacological industries in addition to its use as an ornamental plant. The aim of the experiment was to investigate the effect of cold pre-treatment stress on the callus induction and its subsequent shoot differentiation in ovary culture of marigold cultivar Arka Agni. Ovaries from flower bud pretreated at 4 °c, 7 °c and 25 °c for 1, 24, 72 and 120 hrs were cultured on to callus induction and shoot differentiation media respectively. It was found that 7 0C temperature pre-treatment to be statistically superior for % callus induction (84.58 %), as well 4 0C temperature pre-treatment for minimum days to callus induction (20.30 days) and maximum shoot differentiation rate (10.52 %) followed by 7 0C pre-treatment. Whereas, in case of investigating on duration of exposure of flower buds to different cold pre-treatment, maximum callus induction (89.66 %) was reported from both 7 0C and 4 0C pretreated buds but duration of exposure varies 1hr and 24 hr respectively. However, flower buds pretreated at 4 for 24 hr achieved callus initiation in minimum days (20.00 days) and also resulted in highest shoot differentiation rate (14.15 %). Nevertheless, there is need for testing of the plantlets produced via gynogenesis for genetic variation as well as for the ploidy level.
Pages: 1597-1600 | 304 Views 9 Downloads
How to cite this article:
Thaneshwari, Aswath C, Chaithra N. Effect of cold pre-treatment on callus induction and shoots regeneration in ovary culture of marigold (Tagetes spp). Int J Chem Stud 2018;6(2):1597-1600.