International Journal of Chemical Studies
Vol. 6, Issue 2 (2018)
Genetic diversity in Indian foxtail millet [Setaria italica (L.) P. Beauv] revealed by random amplified polymorphic DNA markers
Author(s): Sumana Sikdar, Sharad Tiwari, Niraj Tripathi and Vishwa Vijay Thakur
Abstract: Sixty five RAPD primers were screened for amplification with 48 foxtail millet [Setaria italica (L.) P. Beauv] genotypes. Successfully amplified 40 primers were selected on the basis of polymorphic banding pattern for final analysis. Selected 40 primers amplified 240 marker loci with 97.54% polymorphism. The band size of amplified markers ranged from 240-3200bp. Average numbers of band per primer were 6.1 while, average numbers of polymorphic band per primer were 5.95. The average Polymorphic Information Content, Effective Marker Ration, Marker Index and Resolving Power values were 0.341, 5.82, 2.08 and 3.16 respectively. In a dendrogram generated by Unweighted Pair Group Analysis 48 genotypes fell into two groups. Two genotypes RFM-84 and TNSI-306 amplified genotype specific amplicons with different primers. These findings not only highlighted the capacity of the RAPD technique for genetic diversity analysis but also help in the selection of diverse S. italica genotypes for conservation and its improvement.
Pages: 1458-1465 | 272 Views 6 Downloads
How to cite this article:
Sumana Sikdar, Sharad Tiwari, Niraj Tripathi, Vishwa Vijay Thakur. Genetic diversity in Indian foxtail millet [Setaria italica (L.) P. Beauv] revealed by random amplified polymorphic DNA markers. Int J Chem Stud 2018;6(2):1458-1465.